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Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Since most cellular processes are mediated by macromolecular assemblies, the systematic identification of protein-protein interactions (PPI) and the identification of the subunit composition of multi-protein complexes can provide insight into gene function and enhance understanding of biological sys...

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Detalhes bibliográficos
Main Authors: Babu, Mohan, Kagan, Olga, Guo, Hongbo, Greenblatt, Jack, Emili, Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3520577/
https://ncbi.nlm.nih.gov/pubmed/23168686
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/4057
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