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Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

PURPOSE: To establish an effective system for isolating primary retinal ganglion cells (RGCs) from newborn mice. METHODS: The retinas were separated from enucleated eyeballs of Crl:CD-1 mice on postnatal day 1 to 4. RGCs were purified using three different methods, including two-step immunopanning (...

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Main Authors: Hong, Samin, Iizuka, Yoko, Kim, Chan Yun, Seong, Gong Je
格式: Artigo
語言:Inglês
出版: Molecular Vision 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3519380/
https://ncbi.nlm.nih.gov/pubmed/23233794
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