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A flexible protein linker improves the function of epitope-tagged proteins in Saccharomyces cerevisiae
Epitope tagging permits the detection of proteins when protein-specific antibodies are not available. However, the epitope tag can reduce the function of the tagged protein. Here we describe a cassette that can be used to introduce an eight amino acid flexible linker between multiple Myc epitopes an...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3518027/ https://ncbi.nlm.nih.gov/pubmed/17192851 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.1431 |
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