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Small epitope-linker modules for PCR-based C-terminal tagging in Saccharomyces cerevisiae

PCR-mediated gene modification is a powerful approach to the functional analysis of genes in Saccharomyces cerevisiae. One application of this method is epitope-tagging of a gene to analyse the corresponding protein by immunological methods. However, the number of epitope tags available in a conveni...

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Hlavní autoři: Funakoshi, Minoru, Hochstrasser, Mark
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley & Sons, Ltd. 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2673350/
https://ncbi.nlm.nih.gov/pubmed/19243080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.1658
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