HyperSpectral imaging microscopy for identification and quantitative analysis of fluorescently-labeled cells in highly autofluorescent tissue

Standard fluorescence microscopy approaches rely on measurements at single excitation and emission bands to identify specific fluorophores and the setting of thresholds to quantify fluorophore intensity. This is often insufficient to reliably resolve and quantify fluorescent labels in tissues due to...

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Bibliografiske detaljer
Main Authors: Leavesley, Silas J., Annamdevula, Naga, Boni, John, Stocker, Samantha, Grant, Kristin, Troyanovsky, Boris, Rich, Thomas C., Alvarez, Diego F.
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3517021/
https://ncbi.nlm.nih.gov/pubmed/21987373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jbio.201100066
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