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HyperSpectral imaging microscopy for identification and quantitative analysis of fluorescently-labeled cells in highly autofluorescent tissue

Standard fluorescence microscopy approaches rely on measurements at single excitation and emission bands to identify specific fluorophores and the setting of thresholds to quantify fluorophore intensity. This is often insufficient to reliably resolve and quantify fluorescent labels in tissues due to...

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Detalhes bibliográficos
Main Authors: Leavesley, Silas J., Annamdevula, Naga, Boni, John, Stocker, Samantha, Grant, Kristin, Troyanovsky, Boris, Rich, Thomas C., Alvarez, Diego F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3517021/
https://ncbi.nlm.nih.gov/pubmed/21987373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jbio.201100066
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