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HyperSpectral imaging microscopy for identification and quantitative analysis of fluorescently-labeled cells in highly autofluorescent tissue

Standard fluorescence microscopy approaches rely on measurements at single excitation and emission bands to identify specific fluorophores and the setting of thresholds to quantify fluorophore intensity. This is often insufficient to reliably resolve and quantify fluorescent labels in tissues due to...

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Détails bibliographiques
Auteurs principaux: Leavesley, Silas J., Annamdevula, Naga, Boni, John, Stocker, Samantha, Grant, Kristin, Troyanovsky, Boris, Rich, Thomas C., Alvarez, Diego F.
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3517021/
https://ncbi.nlm.nih.gov/pubmed/21987373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jbio.201100066
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