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Crystallization, X-ray diffraction analysis and SIRAS phasing of human α-l-iduronidase

Human lysosomal α-l-iduronidase, whose deficiency causes mucopolysaccharid­osis type I, was crystallized using sodium/potassium tartrate and polyethylene glycol 3350 as a precipitant. Using synchrotron radiation, a native data set was collected from a single crystal at 100 K to 2.3 Å resolution. The...

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Detalhes bibliográficos
Main Authors: Maita, Nobuo, Taniguchi, Hisaaki, Sakuraba, Hitoshi
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3515382/
https://ncbi.nlm.nih.gov/pubmed/23143250
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112040432
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