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Measuring incidence angle for through-the-objective total internal reflection fluorescence microscopy
Total internal reflection fluorescence (TIRF) microscopy has the exciting laser beam incident beyond critical angle from the glass side of a glass/aqueous interface formed by the coverslip and aqueous sample. The aqueous side evanescent field decays exponentially with distance from the interface wit...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Society of Photo-Optical Instrumentation Engineers
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3512109/ https://ncbi.nlm.nih.gov/pubmed/23208218 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.17.12.126007 |
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