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Measuring incidence angle for through-the-objective total internal reflection fluorescence microscopy

Total internal reflection fluorescence (TIRF) microscopy has the exciting laser beam incident beyond critical angle from the glass side of a glass/aqueous interface formed by the coverslip and aqueous sample. The aqueous side evanescent field decays exponentially with distance from the interface wit...

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Detalhes bibliográficos
Autor principal: Burghardt, Thomas P.
Formato: Artigo
Idioma:Inglês
Publicado em: Society of Photo-Optical Instrumentation Engineers 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3512109/
https://ncbi.nlm.nih.gov/pubmed/23208218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.17.12.126007
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