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Combined rpoB duplex PCR and hsp65 PCR restriction fragment length polymorphism with capillary electrophoresis as an effective algorithm for identification of Mycobacterial species from clinical isolates

BACKGROUND: Mycobacteria can be quickly and simply identified by PCR restriction-enzyme analysis (PRA), but misidentification can occur because of similarities in band sizes that are critical for discriminating among species. Capillary electrophoresis can provide computer-aided band discrimination....

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Main Authors: Huang, Chen-Cheng, Chen, Jiann-Hwa, Hu, Shiau-Ting, Chiou, Chien-Shun, Huang, Wei-Chang, Hsu, Jeng-Yuan, Lu, Jang-Jih, Shen, Gwan-Han
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3511182/
https://ncbi.nlm.nih.gov/pubmed/22769981
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-12-137
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