Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex-PCR-Based Restriction Analysis and Direct Sequencing

Here we describe a novel duplex PCR method which can differentiate Mycobacterium tuberculosis and nontuberculosis mycobacteria (NTM) strains by amplifying hsp65 DNAs of different sizes (195 and 515 bp, respectively). The devised technique was applied to 54 reference and 170 clinical isolates and dif...

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Detalhes bibliográficos
Main Authors: Kim, Hyun-Ju, Mun, Ho-Suk, Kim, Hong, Oh, Eun-Ju, Ha, Youngju, Bai, Gill-Han, Park, Young-Gil, Cha, Chang-Yong, Kook, Yoon-Hoh, Kim, Bum-Joon
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698348/
https://ncbi.nlm.nih.gov/pubmed/16928964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01214-06
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