Differential Identification of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria by Duplex PCR Assay Using the RNA Polymerase Gene (rpoB)

A novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM), respectively, with two different sets of primers was used to differentially identify 44 reference strains and 379 clinical isolates of mycobacteria in...

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Bibliographic Details
Main Authors: Kim, Bum-Joon, Hong, Seong-Karp, Lee, Keun-Hwa, Yun, Yeo-Jun, Kim, Eui-Chong, Park, Young-Gil, Bai, Gil-Han, Kook, Yoon-Hoh
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Mycobacteriology and Aerobic Actinomycetes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC356837/
https://ncbi.nlm.nih.gov/pubmed/15004105
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.3.1308-1312.2004
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