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Isolation and characterization of mouse and human esophageal epithelial cells in 3D organotypic culture
This protocol describes the isolation and characterization of mouse and human esophageal epithelial cells and the application of 3D organotypic culture (OTC), a form of tissue engineering. This model system permits the interrogation of mechanisms underlying epithelial-stromal interactions. We provid...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3505594/ https://ncbi.nlm.nih.gov/pubmed/22240585 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.437 |
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