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Isolation and characterization of mouse and human esophageal epithelial cells in 3D organotypic culture

This protocol describes the isolation and characterization of mouse and human esophageal epithelial cells and the application of 3D organotypic culture (OTC), a form of tissue engineering. This model system permits the interrogation of mechanisms underlying epithelial-stromal interactions. We provid...

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Bibliografiske detaljer
Main Authors: Kalabis, Jiri, Wong, Gabrielle S, Vega, Maria E, Natsuizaka, Mitsuteru, Robertson, Erle S, Herlyn, Meenhard, Nakagawa, Hiroshi, Rustgi, Anil K
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3505594/
https://ncbi.nlm.nih.gov/pubmed/22240585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.437
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