Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.
The capacity of microsomal membranes to translocate nascent presecretory proteins across their lipid bilayer can be largely abolished by extracting them with high ionic strength buffers. It can be reconstituted by adding the salt extract back to the depleted membranes [Warren, G. & Doberstein, B. (1...
שמור ב:
| הוצא לאור ב: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
National Academy of Sciences
1980
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350451/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6938958/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.12.7112 |
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