Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.
The capacity of microsomal membranes to translocate nascent presecretory proteins across their lipid bilayer can be largely abolished by extracting them with high ionic strength buffers. It can be reconstituted by adding the salt extract back to the depleted membranes [Warren, G. & Doberstein, B. (1...
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| Опубліковано в:: | Proc Natl Acad Sci U S A |
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| Автори: | , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
National Academy of Sciences
1980
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350451/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6938958/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.12.7112 |
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