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Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

The capacity of microsomal membranes to translocate nascent presecretory proteins across their lipid bilayer can be largely abolished by extracting them with high ionic strength buffers. It can be reconstituted by adding the salt extract back to the depleted membranes [Warren, G. & Doberstein, B...

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Bibliografische gegevens
Hoofdauteurs: Walter, P, Blobel, G
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1980
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC350451/
https://ncbi.nlm.nih.gov/pubmed/6938958
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