Specific labeling of the lac carrier protein in membrane vesicles of Escherichia coli by a photoaffinity reagent.

4-Nitrophenyl-alpha-D-galactopyranoside (NPG) was used as a photoaffinity reagent to specifically inactivate the beta-galactoside transport system in Escherichia coli ML 308-225 membrane vesicles. Photolysis of NPG produced time-dependent, irreversible loss of transport activity with corresponding i...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Kaczorowski, G J, LeBlanc, G, Kaback, H R
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1980
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350275/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7005896/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.11.6319
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