Specific labeling of the lac carrier protein in membrane vesicles of Escherichia coli by a photoaffinity reagent.
4-Nitrophenyl-alpha-D-galactopyranoside (NPG) was used as a photoaffinity reagent to specifically inactivate the beta-galactoside transport system in Escherichia coli ML 308-225 membrane vesicles. Photolysis of NPG produced time-dependent, irreversible loss of transport activity with corresponding i...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1980
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350275/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7005896/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.11.6319 |
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