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Specific labeling of the lac carrier protein in membrane vesicles of Escherichia coli by a photoaffinity reagent.

4-Nitrophenyl-alpha-D-galactopyranoside (NPG) was used as a photoaffinity reagent to specifically inactivate the beta-galactoside transport system in Escherichia coli ML 308-225 membrane vesicles. Photolysis of NPG produced time-dependent, irreversible loss of transport activity with corresponding i...

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Detalhes bibliográficos
Main Authors: Kaczorowski, G J, LeBlanc, G, Kaback, H R
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC350275/
https://ncbi.nlm.nih.gov/pubmed/7005896
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