Specific labeling of the lac carrier protein in membrane vesicles of Escherichia coli by a photoaffinity reagent.
4-Nitrophenyl-alpha-D-galactopyranoside (NPG) was used as a photoaffinity reagent to specifically inactivate the beta-galactoside transport system in Escherichia coli ML 308-225 membrane vesicles. Photolysis of NPG produced time-dependent, irreversible loss of transport activity with corresponding i...
Tallennettuna:
| Julkaisussa: | Proc Natl Acad Sci U S A |
|---|---|
| Päätekijät: | , , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
National Academy of Sciences
1980
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350275/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7005896/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.11.6319 |
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