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Probing exchange kinetics and atomic resolution dynamics in high-molecular-weight complexes using dark-state exchange saturation transfer NMR spectroscopy
We present the protocol for the measurement and analysis of dark-state exchange saturation transfer (DEST), a novel solution NMR method for characterizing, at atomic resolution, the interaction between an NMR-‘visible’ free species and an NMR-‘invisible’ species transiently bound to a very high-mole...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3500623/ https://ncbi.nlm.nih.gov/pubmed/22814391 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.077 |
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