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Crosslinkage and visualization of acetylcholine receptors on myotubes with biotinylated alpha-bungarotoxin and fluorescent avidin.

A biotinylated derivative of alpha-bungarotoxin and tetramethylrhodamine-labeled avidin were employed to fluorescence label the acetylcholine receptors (AcChoR) on the surface of rat myotubes in primary culture. Because of the multivalency of both the biotinylated bungarotoxin and the avidin, this t...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteur principal: Axelrod, D
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1980
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349939/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6933533/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.8.4823
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