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Transfer in SDS of biotinylated proteins from acrylamide gels to an avidin-coated membrane filter

Avidin was covalently linked to aldehyde-derivatized polyethersulfone membrane filters. These filters were used in Western blot analysis of proteins reacted with biotinylation reagents and electrophoresed in sodium dodecyl sulfate (SDS) on polyacrylamide gels. Electrophoretic transfer from the gels...

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Detalles Bibliográficos
Principais autores: Arthur Karlin, Chaojian Wang, Jing Li, Qiang Xu
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis Group 2004-06-01
Series:BioTechniques
Acceso en liña:https://www.future-science.com/doi/10.2144/04366RR02
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