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Transfer in SDS of biotinylated proteins from acrylamide gels to an avidin-coated membrane filter

Avidin was covalently linked to aldehyde-derivatized polyethersulfone membrane filters. These filters were used in Western blot analysis of proteins reacted with biotinylation reagents and electrophoresed in sodium dodecyl sulfate (SDS) on polyacrylamide gels. Electrophoretic transfer from the gels...

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Hlavní autoři: Arthur Karlin, Chaojian Wang, Jing Li, Qiang Xu
Médium: Artigo
Jazyk:Inglês
Vydáno: Taylor & Francis Group 2004-06-01
Edice:BioTechniques
On-line přístup:https://www.future-science.com/doi/10.2144/04366RR02
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