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An efficient method for variable region assembly in the construction of scFv phage display libraries using independent strand amplification
Phage display library technology is a common method to produce human antibodies. In this technique, the immunoglobulin variable regions are displayed in a bacteriophage in a way that each filamentous virus displays the product of a single antibody gene on its surface. From the collection of differen...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Landes Bioscience
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3499348/ https://ncbi.nlm.nih.gov/pubmed/22692130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.20653 |
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