An efficient method for variable region assembly in the construction of scFv phage display libraries using independent strand amplification

Phage display library technology is a common method to produce human antibodies. In this technique, the immunoglobulin variable regions are displayed in a bacteriophage in a way that each filamentous virus displays the product of a single antibody gene on its surface. From the collection of differen...

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Main Authors: Sotelo, Pablo H., Collazo, Noberto, Zuñiga, Roberto, Gutiérrez-González, Matías, Catalán, Diego, Ribeiro, Carolina Hager, Aguillón, Juan Carlos, Molina, María Carmen
Format: Artigo
Language:Inglês
Published: Landes Bioscience 2012
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3499348/
https://ncbi.nlm.nih.gov/pubmed/22692130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.20653
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