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Co-transcriptional Assembly of Chemically Modified RNA Nanoparticles Functionalized with siRNAs

[Image: see text] We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2′-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatica...

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Bibliographic Details
Main Authors: Afonin, Kirill A., Kireeva, Maria, Grabow, Wade W., Kashlev, Mikhail, Jaeger, Luc, Shapiro, Bruce A.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3498980/
https://ncbi.nlm.nih.gov/pubmed/23016824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nl302302e
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