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Expression, purification, crystallization and preliminary X-ray analysis of ribitol-5-phosphate cytidylyltransferase from Bacillus subtilis

TarI is a ribitol-5-phosphate cytidylyltransferase that catalyzes the formation of CDP-ribitol, which is involved in the biosynthesis of wall teichoic acids, from CTP and ribitol 5-phosphate. TarI from Bacillus subtilis (BsTarI) was purified and crystallized using the sitting-drop vapour-diffusion m...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Chen, Sheng-Chia, Yang, Chia Shin, Lin, Ching-Ting, Chan, Nei-Li, Chang, Ming-Chung, Chen, Yeh
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3497978/
https://ncbi.nlm.nih.gov/pubmed/23027746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112035142
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