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Temperature dependence of protein folding kinetics in living cells

We measure the stability and folding rate of a mutant of the enzyme phosphoglycerate kinase (PGK) inside bone tissue cells as a function of temperature from 38 to 48 °C. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring...

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Bibliografische gegevens
Hoofdauteurs: Guo, Minghao, Xu, Yangfan, Gruebele, Martin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2012
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3497798/
https://ncbi.nlm.nih.gov/pubmed/22665776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201797109
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