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Temperature dependence of protein folding kinetics in living cells
We measure the stability and folding rate of a mutant of the enzyme phosphoglycerate kinase (PGK) inside bone tissue cells as a function of temperature from 38 to 48 °C. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3497798/ https://ncbi.nlm.nih.gov/pubmed/22665776 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1201797109 |
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