Chromosome-mediated gene transfer results in two classes of unstable transformants.

The human thymidine kinase gene has been transferred from HeLa S3 cells to mouse LM(TK-) cells via isolated metaphase chromosomes. Efficient transfer of the thymidine kinase gene (1.8 X 10(-5) colonies per recipient cell) was obtained when the donor chromosomes were precipitated with calcium phospha...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Klobutcher, L A, Miller, C L, Ruddle, F H
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1980
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349667/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6932038/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.6.3610
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