Catalytic mechanism of serine proteases: reexamination of the pH dependence of the histidyl 1J13C2-H coupling constant in the catalytic triad of alpha-lytic protease.

L-Histidine, 90% 13C enriched at the C2 position, was incorporated into the catalytic triad of alpha-lytic protease (EC 3.4.21.12) with the aid of histidine-requiring mutant of Lysobacter enzymogenes (ATC 29487), and the pH dependence of the coupling constant between this carbon atom and its directl...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Bachovchin, W W, Kaiser, R, Richards, J H, Roberts, J D
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1981
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349258/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7038675/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.12.7323
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