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Counting single photoactivatable fluorescent molecules by photoactivated localization microscopy (PALM)
We present a single molecule method for counting proteins within a diffraction-limited area when using photoactivated localization microscopy. The intrinsic blinking of photoactivatable fluorescent proteins mEos2 and Dendra2 leads to an overcounting error, which constitutes a major obstacle for thei...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3491528/ https://ncbi.nlm.nih.gov/pubmed/23045631 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1215175109 |
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