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Counting single photoactivatable fluorescent molecules by photoactivated localization microscopy (PALM)

We present a single molecule method for counting proteins within a diffraction-limited area when using photoactivated localization microscopy. The intrinsic blinking of photoactivatable fluorescent proteins mEos2 and Dendra2 leads to an overcounting error, which constitutes a major obstacle for thei...

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Detalhes bibliográficos
Main Authors: Lee, Sang-Hyuk, Shin, Jae Yen, Lee, Antony, Bustamante, Carlos
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3491528/
https://ncbi.nlm.nih.gov/pubmed/23045631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1215175109
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