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Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
Protein-protein interactions (PPIs) are key molecular events to biology. However, it remains a challenge to visualize PPIs with sufficient resolution and sensitivity in cells because the resolution of conventional light microscopy is diffraction-limited to ~250 nm. By combining bimolecular fluoresce...
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Udgivet i: | J Vis Exp |
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Main Authors: | , , , |
Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
MyJove Corporation
2015
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4758764/ https://ncbi.nlm.nih.gov/pubmed/26779930 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53154 |
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