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Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Protein-protein interactions (PPIs) are key molecular events to biology. However, it remains a challenge to visualize PPIs with sufficient resolution and sensitivity in cells because the resolution of conventional light microscopy is diffraction-limited to ~250 nm. By combining bimolecular fluoresce...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Nickerson, Andrew, Huang, Tao, Lin, Li-Jung, Nan, Xioalin
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4758764/
https://ncbi.nlm.nih.gov/pubmed/26779930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53154
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