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Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries
Site-directed mutagenesis is routinely performed in protein engineering experiments. One method, termed Kunkel mutagenesis, is frequently used for constructing libraries of peptide or protein variants in M13 bacteriophage, followed by affinity selection of phage particles. To make this method more e...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3491174/ https://ncbi.nlm.nih.gov/pubmed/22959950 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.08.008 |
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