A Novel Helper Phage Enabling Construction of Genome-Scale ORF-Enriched Phage Display Libraries

Phagemid-based expression of cloned genes fused to the gIIIP coding sequence and rescue using helper phages, such as VCSM13, has been used extensively for constructing large antibody phage display libraries. However, for randomly primed cDNA and gene fragment libraries, this system encounters readin...

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Autori principali: Gupta, Amita, Shrivastava, Nimisha, Grover, Payal, Singh, Ajay, Mathur, Kapil, Verma, Vaishali, Kaur, Charanpreet, Chaudhary, Vijay K.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3785514/
https://ncbi.nlm.nih.gov/pubmed/24086469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0075212
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