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A Bioinformatics Method Identifies Prominent Off-targeted Transcripts in RNAi Screens

Because off-target effects hamper interpretation and validation of RNAi screens, we developed a bioinformatics method, Genome-wide Enrichment of Seed Sequence matches (GESS), to identify candidate off-targeted transcripts from direct analysis of primary screening data. GESS identified a prominent of...

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Hlavní autoři: Sigoillot, Frederic D., Lyman, Susan, Huckins, Jeremy F., Adamson, Britt, Chung, Eunah, Quattrochi, Brian, King, Randall W.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3482495/
https://ncbi.nlm.nih.gov/pubmed/22343343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1898
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