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Characterization of HIV Tat modifications using novel methyl-lysine-specific antibodies

Modification-specific antibodies are important tools to examine the dynamics and functions of posttranslational protein modifications in cells. Here, we describe in detail the generation of polyclonal antibodies specific for mono-, di- and trimethylated lysine 51 within the HIV transactivator Tat. L...

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Bibliographic Details
Main Authors: Pagans, Sara, Sakane, Naoki, Schnoelzer, Martina, Ott, Melanie
Format: Artigo
Language:Inglês
Published: 2010
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3478124/
https://ncbi.nlm.nih.gov/pubmed/20615470
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.07.001
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