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On-line amino acid-based capillary isoelectric focusing-ESI-MS/MS for protein digests analysis
Six amino acids with pIs that ranged from 3.2 to 9.7 were used as ampholytes to establish a pH gradient in capillary isoelectric focusing. This amino acid-based capillary isoelectric focusing (cIEF) was coupled with ESI-MS/MS using an electrokinetically pumped sheath-flow interface for peptide analy...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3474973/ https://ncbi.nlm.nih.gov/pubmed/23062442 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.aca.2012.04.026 |
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