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Capillary Isoelectric Focusing-Tandem Mass Spectrometry And Reversed-Phase Liquid Chromatography-Tandem Mass Spectrometry For Quantitative Proteomic Analysis Of Differentiating PC12 Cells By Eight-Plex iTRAQ

We report the application of capillary isoelectric focusing for quantitative analysis of a complex proteome. Biological duplicates were generated from PC12 cells at days 0, 3, 7, and 12 following treatment with nerve growth factor. These biological duplicates were digested with trypsin, labeled usin...

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Detalhes bibliográficos
Main Authors: Zhu, Guijie, Sun, Liangliang, Keithley, Richard B., Dovichi, Norman J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3759156/
https://ncbi.nlm.nih.gov/pubmed/23822771
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac4009868
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