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Integrated CZE-ESI-MS/MS system with an immobilized trypsin microreactor for online digestion and analysis of picogram amounts of RAW 264.7 cell lysate

A capillary zone electrophoresis (CZE) electrospray ionization (ESI) tandem mass spectrometry (MS/MS) system was integrated with an immobilized trypsin microreactor. The system was evaluated and then applied for online digestion and analysis of picogram loadings of RAW 264.7 cell lysate. Protein sam...

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Main Authors: Sun, Liangliang, Zhu, Guijie, Dovichi, Norman J.
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635492/
https://ncbi.nlm.nih.gov/pubmed/23510126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac400523x
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