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Isolated-patch recording from liposomes containing functionally reconstituted chloride channels from Torpedo electroplax.

Small unilamellar vesicles formed from purified phospholids by detergent/dialysis methods may be enlarged to 30-microns diameter by freezing and thawing. Very-high-resistance seals were formed by applying a glass micropipette to the surface of these large liposomes, and single bilayer "patches&...

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Autors principals: Tank, D W, Miller, C, Webb, W W
Format: Artigo
Idioma:Inglês
Publicat: 1982
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC347425/
https://ncbi.nlm.nih.gov/pubmed/6296849
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