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Isolated-patch recording from liposomes containing functionally reconstituted chloride channels from Torpedo electroplax.

Small unilamellar vesicles formed from purified phospholids by detergent/dialysis methods may be enlarged to 30-microns diameter by freezing and thawing. Very-high-resistance seals were formed by applying a glass micropipette to the surface of these large liposomes, and single bilayer "patches" of m...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Tank, D W, Miller, C, Webb, W W
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1982
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347425/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6296849/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.24.7749
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