Isolated-patch recording from liposomes containing functionally reconstituted chloride channels from Torpedo electroplax.
Small unilamellar vesicles formed from purified phospholids by detergent/dialysis methods may be enlarged to 30-microns diameter by freezing and thawing. Very-high-resistance seals were formed by applying a glass micropipette to the surface of these large liposomes, and single bilayer "patches" of m...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1982
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347425/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6296849/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.24.7749 |
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