Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion

Genetically encoded phosphoserine incorporation programmed by the UAG codon was achieved by addition of engineered elongation factor and an archaeal aminoacyl-tRNA synthetase to the normal Escherichia coli translation machinery (Park (2011) Science 333, 1151). However, protein yield suffers from exp...

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Hlavní autoři: Heinemann, Ilka U., Rovner, Alexis J., Aerni, Hans R., Rogulina, Svetlana, Cheng, Laura, Olds, William, Fischer, Jonathan T., Söll, Dieter, Isaacs, Farren J., Rinehart, Jesse
Médium: Artigo
Jazyk:Inglês
Vydáno: 2012
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3473164/
https://ncbi.nlm.nih.gov/pubmed/22982858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.febslet.2012.08.031
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