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Replacement of anticodon loop nucleotides to produce functional tRNAs: amber suppressors derived from yeast tRNAPhe.

The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe. By constructing tRNAs with a CUA anticodon, complementary to the amber (UAG) terminator, functional amber suppressor tRNAs were produced. The activity of these tRNAs was assayed in a mammalian cell-free prot...

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Autores principales: Bruce, A G, Atkins, J F, Wills, N, Uhlenbeck, O, Gesteland, R F
Formato: Artigo
Lenguaje:Inglês
Publicado: 1982
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC347291/
https://ncbi.nlm.nih.gov/pubmed/6961400
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