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Replacement of anticodon loop nucleotides to produce functional tRNAs: amber suppressors derived from yeast tRNAPhe.

The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe. By constructing tRNAs with a CUA anticodon, complementary to the amber (UAG) terminator, functional amber suppressor tRNAs were produced. The activity of these tRNAs was assayed in a mammalian cell-free prot...

詳細記述

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書誌詳細
出版年:Proc Natl Acad Sci U S A
主要な著者: Bruce, A G, Atkins, J F, Wills, N, Uhlenbeck, O, Gesteland, R F
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 1982
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347291/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6961400/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.23.7127
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