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Replacement of anticodon loop nucleotides to produce functional tRNAs: amber suppressors derived from yeast tRNAPhe.

The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe. By constructing tRNAs with a CUA anticodon, complementary to the amber (UAG) terminator, functional amber suppressor tRNAs were produced. The activity of these tRNAs was assayed in a mammalian cell-free prot...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Bruce, A G, Atkins, J F, Wills, N, Uhlenbeck, O, Gesteland, R F
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347291/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6961400/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.23.7127
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