Replacement of anticodon loop nucleotides to produce functional tRNAs: amber suppressors derived from yeast tRNAPhe.
The method of anticodon loop replacement has been used to make derivatives of yeast tRNAPhe. By constructing tRNAs with a CUA anticodon, complementary to the amber (UAG) terminator, functional amber suppressor tRNAs were produced. The activity of these tRNAs was assayed in a mammalian cell-free prot...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
|---|---|
| Principais autores: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1982
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347291/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6961400/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.23.7127 |
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