Site-directed psoralen crosslinking of DNA.
A technique has been developed for placing site-specific crosslinks into DNA by using a psoralen derivative containing a sulfhydryl group. Plasmid pBR322 was nicked at the unique BamHI restriction site, and mercurated pyrimidines were introduced by nick-translation. Then the psoralen was specificall...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1982
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346721/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6289307/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.15.4594 |
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